COS-7 cells were transfected for 24 hrs with a plasmid expressing FLAG-ERM (left panels) or FLAG-ERM KR12345 (right panels) . Untreated (-) and H2O2-treated (+) cells were collected for immunoblot analysis. Top panels : cell lysates probed by western blot (WB) with an anti-ERM antibody. Center panels : cell lysates immunoprecipitated (IP) with an anti-FLAG antibody followed by WB with SUMO1 polyclonal antibody (Cat # PAB2402) . Bottom panels : cell lysates immunoprecipitated with an anti-FLAG antibody followed by WB with SUMO2/3 polyclonal antibody (Cat # PAB2408) . (*) represents immunoprecipitated ERM-like forms recognized by anti-SUMO antibodies.
Western Blot (Recombinant protein)
The SUMO1 polyclonal antibody (Cat # PAB2402) is used in Western blot to detect GST-SUMO1 fusion protein.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the SUMO1 polyclonal antibody (Cat # PAB2402) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq
GAP modifying protein 1,SMT3 suppressor of mif two 3 homolog 1,sentrin,ubiquitin-like 1 (sentrin)