PRMT8 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of PRMT8 polyclonal antibody (Cat # PAB2400) in K-562 cell line lysates (35 ug/lane). PRMT8 (arrow) was detected using the purified polyclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of PRMT8 (arrow) using rabbit PRMT8 polyclonal antibody (Cat # PAB2400). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRMT8 gene (Lane 2) (Origene Technologies).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PRMT8.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human PRMT8.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of PRMT8 polyclonal antibody (Cat # PAB2400) in K-562 cell line lysates (35 ug/lane). PRMT8 (arrow) was detected using the purified polyclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of PRMT8 (arrow) using rabbit PRMT8 polyclonal antibody (Cat # PAB2400). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRMT8 gene (Lane 2) (Origene Technologies). -
Gene Info — PRMT8
Entrez GeneID
56341Protein Accession#
NP_062828;Q9NR22Gene Name
PRMT8
Gene Alias
HRMT1L3, HRMT1L4
Gene Description
protein arginine methyltransferase 8
Omim ID
610086Gene Ontology
HyperlinkGene Summary
Arginine methylation is a widespread posttranslational modification mediated by arginine methyltransferases, such as PRMT8. Arginine methylation is involved in a number of cellular processes, including DNA repair, RNA transcription, signal transduction, protein compartmentalization, and possibly protein translation (Lee et al., 2005 [PubMed 16051612]).[supplied by OMIM
Other Designations
HMT1 hnRNP methyltransferase-like 3|HMT1 hnRNP methyltransferase-like 4|protein arginine N-methyltransferase 4|protein arginine N-methyltransferase 8
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Interactome
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Disease
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Publication Reference
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PRMT8, a new membrane-bound tissue-specific member of the protein arginine methyltransferase family.
Lee J, Sayegh J, Daniel J, Clarke S, Bedford MT.
The Journal of Biological Chemistry 2005 Sep; 280(38):32890.
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PRMT8, a new membrane-bound tissue-specific member of the protein arginine methyltransferase family.
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