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Last updated: 2020/5/24
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PRDM14 polyclonal antibody 

  • Catalog # : PAB2396
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of PRDM14.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PRDM14.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • Western Blot (1:1000)
    Immunohistochemistry (1:50-100)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of PRDM14 polyclonal antibody (Cat # PAB2396) in A-549 cell lysate. PRDM14 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human cancer tissue reacted with the PRDM14 polyclonal antibody (Cat # PAB2396) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma ; HC = hepatocarcinoma.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of PRDM14 in breast cancer cells. SK-BR-3 cells were transfected with pCMV-Tag2A or pCMV-PRDM14 and then labeled with PRDM14 polyclonal antibody (Cat # PAB2396). The nucleus was stained with DAPI.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Protein Accession#:
  • NP_078780;Q9GZV8
  • Gene Name:
  • PRDM14
  • Gene Alias:
  • MGC59730,PFM11
  • Gene Description:
  • PR domain containing 14
  • Gene Summary:
  • The PR domain is a protein-protein interaction module of about 100 amino acids. PR domain-containing proteins, such as PRDM14, are often involved in transcriptional regulation (Jiang and Huang, 2000 [PubMed 10668202]).[supplied by OMIM
  • Other Designations:
  • PR-domain zinc finger protein 14
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