CENPE polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant CENPE.
Immunogen
Recombinant protein corresponding to amino acids of human CENPE.
Sequence
EKIENLTRMLVTSSSLTLQQELKAKRKRRVTWCLGKINKMKNSNYADQFNIPTNITTKTHKLSINLLREIDESVCSESDVFSNTLDTLSEIEWNPATKLLNQENIESELNSLRADYDN
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:1000-1:2500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human stomach with CENPE polyclonal antibody (Cat # PAB23920) shows strong nuclear positivity in glandular cells.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with CENPE polyclonal antibody (Cat # PAB23920) at 1-4 ug/mL dilution shows positivity in cytoplasm and cytoskeleton (microtubules). -
Gene Info — CENPE
Entrez GeneID
1062Protein Accession#
Q02224Gene Name
CENPE
Gene Alias
KIF10
Gene Description
centromere protein E, 312kDa
Omim ID
117143Gene Ontology
HyperlinkGene Summary
Centrosome-associated protein E is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. CENPE is proposed to be one of the motors responsible for mammalian chromosome movement and/or spindle elongation. [provided by RefSeq
Other Designations
Centromere autoantigen E (312kD)|centromere protein E|centromere protein E (312kD)|kinesin family member 10
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Interactome
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Disease
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