DCTN5 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant DCTN5.
Immunogen
Recombinant protein corresponding to amino acids of human DCTN5.
Sequence
LGELLYNKSEYIETASGNKVSRQSVLCGSQNIVLNGKTIVMNDCIIRGDLANVRVGRHCVVKSRSVIRPPFKKFSKGV
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:10-1:20)
Western Blot (1:250-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with DCTN5 polyclonal antibody (Cat # PAB23889).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human colon with DCTN5 polyclonal antibody (Cat # PAB23889) shows strong cytoplasmic positivity in glandular cells. -
Gene Info — DCTN5
Entrez GeneID
84516Protein Accession#
Q9BTE1Gene Name
DCTN5
Gene Alias
MGC3248, p25
Gene Description
dynactin 5 (p25)
Gene Ontology
HyperlinkGene Summary
Dynactin (see MIM 601143) is a multimeric protein essential for minus-end-directed transport driven by the microtubule-based motor dynein (see DYNC1H1; MIM 600112). DCTN5 is a subunit of the pointed-end subcomplex of dynactin that is thought to interact with membranous cargo (Parisi et al., 2004 [PubMed 15043994]).[supplied by OMIM
Other Designations
dynactin 4|dynactin 5
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Interactome
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Disease
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Publication Reference
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Conformational diversity of dynactin sidearm and domain organization of its subunit p150.
Saito K, Murayama T, Hata T, Kobayashi T, Shibata K, Kazuno S, Fujimura T, Sakurai T, Toyoshima YY.
Molecular Biology of the Cell 2020 Jun; 31(12):1218.
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Conformational diversity of dynactin sidearm and domain organization of its subunit p150.
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