POU1F1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant POU1F1.
Immunogen
Recombinant protein corresponding to amino acids of human POU1F1.
Sequence
CKLKAILSKWLEEAEQVGALYNEKVGANERKRKRRTTISIAAKDALERHFGEQNKPSSQEIMRMAE
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:500-1:1000)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human vagina with POU1F1 polyclonal antibody (Cat # PAB23836) shows moderate nuclear positivity in squamous epithelial cells.Immunofluorescence
Immunofluorescent staining of human cell line A-431 with POU1F1 polyclonal antibody (Cat # PAB23836) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli and cytoplasm. -
Gene Info — POU1F1
Entrez GeneID
5449Protein Accession#
P28069Gene Name
POU1F1
Gene Alias
GHF-1, PIT1, Pit-1
Gene Description
POU class 1 homeobox 1
Omim ID
173110Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the POU family of transcription factors that regulate mammalian development. The protein regulates expression of several genes involved in pituitary development and hormone expression. Mutations in this genes result in combined pituitary hormone deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
POU domain, class 1, transcription factor 1|growth hormone factor 1|pituitary specific transcription factor 1|pituitary-specific transcription factor 1
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Interactome
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Disease
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