Prdm2 polyclonal antibody
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More Files
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Immunocytochemistry
Immunocytochemical analysis of Prdm2 in adenovirus-transduced tumor cells. Established HCT-116 xenograft tumors at day 2 after infection of AdRIZ1, Adnull, or buffer alone were processed for RIZ1 in situ detection (TUNEL) analysis.
Immunoprecipitation
Rat B50 brain tumor cell extracts were immunoprecipitated with Prdm2 polyclonal antibody (Cat # PAB2383) or preimmune.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant Prdm2.
Immunogen
Recombinant GST fusion protein corresponding to amino acids 245-573 of rat Prdm2.
Host
Rabbit
Theoretical MW (kDa)
185
Reactivity
Human, Mouse, Rat
Specificity
This antibody also recognizes the isoform beta (Riz2) of PRDM2 (185 KDa).
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (1:100-500)
Immunoprecipitation
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry
Immunocytochemistry
Immunocytochemical analysis of Prdm2 in adenovirus-transduced tumor cells. Established HCT-116 xenograft tumors at day 2 after infection of AdRIZ1, Adnull, or buffer alone were processed for RIZ1 in situ detection (TUNEL) analysis.Immunoprecipitation
Rat B50 brain tumor cell extracts were immunoprecipitated with Prdm2 polyclonal antibody (Cat # PAB2383) or preimmune. -
Gene Info — Prdm2
Entrez GeneID
313678GeneBank Accession#
NM_012231;NM_015866Gene Name
Prdm2
Gene Alias
Riz
Gene Description
PR domain containing 2, with ZNF domain
Gene Ontology
HyperlinkGene Summary
O
Other Designations
retinoblastoma interacting zinc finger protein|retinoblastoma protein-binding zinc finger protein|zinc finger protein RIZ
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Publication Reference
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cDNA cloning of a novel protein containing two zinc-finger domains that may function as a transcription factor for the human heme-oxygenase-1 gene.
Muraosa Y, Takahashi K, Yoshizawa M, Shibahara S.
European Journal of Biochemistry 1996 Feb; 235(3):471.
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The retinoblastoma protein binds to RIZ, a zinc-finger protein that shares an epitope with the adenovirus E1A protein.
Buyse IM, Shao G, Huang S.
PNAS 1995 May; 92(10):4467.
Application：IP, Human, Rat, HT-1080 cells, Rat brains.
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cDNA cloning of a novel protein containing two zinc-finger domains that may function as a transcription factor for the human heme-oxygenase-1 gene.
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