KATNB1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant KATNB1.
Immunogen
Recombinant protein corresponding to amino acids of human KATNB1.
Sequence
SPAMDVQFPVPNLEVLPRPPVVASTPAPKAEPAIIPATRNEPIGLKASDFLPAVKIPQQAELVDEDAMSQIRKGHDTMCVVLTSRHKNLDTVRAVWTMGDIKTSVDSAVAINDLSVV
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:1000-1:2500)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with KATNB1 polyclonal antibody (Cat # PAB23761) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human stomach with KATNB1 polyclonal antibody (Cat # PAB23761) shows strong cytoplasmic positivity in glandular cells at 1:1000-1:2500 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with KATNB1 polyclonal antibody (Cat # PAB23761) at 1-4 ug/mL dilution shows positivity in plasma membrane, cytoplasm and cytoskeleton (microtubules). -
Gene Info — KATNB1
Entrez GeneID
10300Protein Accession#
Q9BVA0Gene Name
KATNB1
Gene Alias
KAT
Gene Description
katanin p80 (WD repeat containing) subunit B 1
Omim ID
602703Gene Ontology
HyperlinkGene Summary
Microtubules, polymers of alpha and beta tubulin subunits, form the mitotic spindle of a dividing cell and help to organize membranous organelles during interphase. Katanin is a heterodimer that consists of a 60 kDa ATPase (p60 subunit A 1) and an 80 kDa accessory protein (p80 subunit B 1). The p60 subunit acts to sever and disassemble microtubules, while the p80 subunit targets the enzyme to the centrosome. Katanin is a member of the AAA family of ATPases. [provided by RefSeq
Other Designations
katanin (80 kDa)|katanin p80 (WD40-containing) subunit B 1|katanin p80 subunit B 1
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