ADO polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate).
Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with ADO polyclonal antibody (Cat # PAB23658).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cerebral cortex with ADO polyclonal antibody (Cat # PAB23658) shows strong cytoplasmic positivity in neuronal cells.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant ADO.
Immunogen
Recombinant protein corresponding to amino acids of human ADO.
Sequence
GASDRDAASGPEAPMQPGFPENLSKLKSLLTQLRAEDLNIAPRKATLQPL
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:10-1:20)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate).
Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with ADO polyclonal antibody (Cat # PAB23658).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cerebral cortex with ADO polyclonal antibody (Cat # PAB23658) shows strong cytoplasmic positivity in neuronal cells. -
Gene Info — ADO
Entrez GeneID
84890Protein Accession#
Q96SZ5Gene Name
ADO
Gene Alias
C10orf22, DKFZp564C046, FLJ14547
Gene Description
2-aminoethanethiol (cysteamine) dioxygenase
Omim ID
611392Gene Ontology
HyperlinkGene Summary
Human thiol dioxygenases include cysteine dioxygenase (CDO; MIM 603943) and cysteamine (2-aminoethanethiol) dioxygenase (ADO; EC 1.13.11.19). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine (Dominy et al., 2007 [PubMed 17581819]).[supplied by OMIM
Other Designations
OTTHUMP00000062121|cysteamine (2-aminoethanethiol) dioxygenase (ADO)|cysteamine dioxygenase
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Interactome
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Pathway
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