PIGN polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant PIGN.
Immunogen
Recombinant protein corresponding to amino acids of human PIGN.
Sequence
KVDDGVKEIVSMFNHFYGNDGKTTFIFTSDHGMTDWGSHGAGHPSETLTPLVTWGAGIKYPQRVSAQQFDDAFLKEWRLENWK
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:200-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate).
Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with PIGN polyclonal antibody (Cat # PAB23650).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human stomach with PIGN polyclonal antibody (Cat # PAB23650) shows strong cytoplasmic positivity in glandular cells at 1:200-1:500 dilution. -
Gene Info — PIGN
Entrez GeneID
23556Protein Accession#
O95427Gene Name
PIGN
Gene Alias
MCD4, MDC4, MGC26427, PIG-N
Gene Description
phosphatidylinositol glycan anchor biosynthesis, class N
Omim ID
606097Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is expressed in the endoplasmic reticulum and transfers phosphoethanolamine (EtNP) to the first mannose of the GPI anchor. Two alternatively spliced variants, which encode an identical isoform, have been reported. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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Disease
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