IFITM3 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of IFITM3 polyclonal antibody (Cat # PAB2360) in 293 cell line lysates (35 ug/lane). IFITM3 (arrow) was detected using the purified polyclonal antibody.
Immunofluorescence
HeLa cells stained with IFITM3 polyclonal antibody (Cat # PAB2360), without (A) or with (B) blocking peptide (P0250).
Immunofluorescence
WI-38 (primary human fibroblasts) treated with IFITM3 polyclonal antibody (Cat # PAB2360)(Red).
Secondary: Alexafluor 568. Cellular membranes stained with wheat germ agglutinin (orange).
Microtubules stained with phalloidin (green).
Nuclei stained with DAPI (blue).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of IFITM3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human IFITM3.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
Immunofluorescence (1:100-500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of IFITM3 polyclonal antibody (Cat # PAB2360) in 293 cell line lysates (35 ug/lane). IFITM3 (arrow) was detected using the purified polyclonal antibody.Immunofluorescence
HeLa cells stained with IFITM3 polyclonal antibody (Cat # PAB2360), without (A) or with (B) blocking peptide (P0250).Immunofluorescence
WI-38 (primary human fibroblasts) treated with IFITM3 polyclonal antibody (Cat # PAB2360)(Red).
Secondary: Alexafluor 568. Cellular membranes stained with wheat germ agglutinin (orange).
Microtubules stained with phalloidin (green).
Nuclei stained with DAPI (blue). -
Gene Info — IFITM3
Entrez GeneID
10410Protein Accession#
NP_066362;Q01628Gene Name
IFITM3
Gene Alias
1-8U, IP15
Gene Description
interferon induced transmembrane protein 3 (1-8U)
Omim ID
605579Gene Ontology
HyperlinkOther Designations
interferon-induced transmembrane protein 3 (1-8U)|interferon-inducible
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Interactome
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Disease
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Publication Reference
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IFITM/Mil/fragilis family proteins IFITM1 and IFITM3 play distinct roles in mouse primordial germ cell homing and repulsion.
Tanaka SS, Yamaguchi YL, Tsoi B, Lickert H, Tam PP.
Developmental Cell 2005 Dec; 9(6):745.
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IFITM/Mil/fragilis family proteins IFITM1 and IFITM3 play distinct roles in mouse primordial germ cell homing and repulsion.
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