KIF18A polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant KIF18A.
Immunogen
Recombinant protein corresponding to amino acids of human KIF18A.
Sequence
LHCHHLHLQNKDLKAQIRHMMDLACLQEQQHRQTEAVLNALLPTLRKQYCTLKEAGLSNAAFESDFKEIEHLVERKKVVVWADQTAEQPKQND
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:20-1:50)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with KIF18A polyclonal antibody (Cat # PAB23531) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human rectum with KIF18A polyclonal antibody (Cat # PAB23531) shows strong cytoplasmic positivity in glandular cells at 1:20-1:50 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with KIF18A polyclonal antibody (Cat # PAB23531) at 1-4 ug/mL dilution shows positivity in cytoplasm and cytoskeleton (microtubules). -
Gene Info — KIF18A
Entrez GeneID
81930Protein Accession#
Q8NI77Gene Name
KIF18A
Gene Alias
DKFZp434G2226, MS-KIF18A
Gene Description
kinesin family member 18A
Gene Ontology
HyperlinkGene Summary
KIF18A is a member of the kinesin superfamily of microtubule-associated molecular motors (see MIM 148760) that use hydrolysis of ATP to produce force and movement along microtubules (Luboshits and Benayahu, 2005 [PubMed 15878648]).[supplied by OMIM
Other Designations
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