ELP3 polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot analysis of ELP3 (arrow) using rabbit ELP3 polyclonal antibody (Cat # PAB2345). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ELP3 gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human testis tissue reacted with ELP3 polyclonal antibody (Cat # PAB2345) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ELP3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human ELP3.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of ELP3 (arrow) using rabbit ELP3 polyclonal antibody (Cat # PAB2345). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ELP3 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human testis tissue reacted with ELP3 polyclonal antibody (Cat # PAB2345) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. -
Gene Info — ELP3
Entrez GeneID
55140Protein Accession#
NP_060561;Q9H9T3Gene Name
ELP3
Gene Alias
FLJ10422, KAT9
Gene Description
elongation protein 3 homolog (S. cerevisiae)
Gene Ontology
HyperlinkGene Summary
ELP3 is the catalytic subunit of the histone acetyltransferase elongator complex, which contributes to transcript elongation and also regulates the maturation of projection neurons (Creppe et al., 2009 [PubMed 19185337]).[supplied by OMIM
Other Designations
elongation protein 3 homolog
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Interactome
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Disease
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Publication Reference
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hELP3 subunit of the Elongator complex regulates the transcription of HSP70 gene in human cells.
Han Q, Hou X, Su D, Pan L, Duan J, Cui L, Huang B, Lu J.
Acta Biochimica et Biophysica Sinica 2007 Jun; 39(6):453.
Application：ELISA, WB-Tr, Human, HEK 293T cells.
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Human Elongator facilitates RNA polymerase II transcription through chromatin.
Kim JH, Lane WS, Reinberg D.
PNAS 2002 Feb; 99(3):1241.
Application：IF, IP, WB-Re, Human, HeLa cells, Recombinant protein.
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Genomic organization and isoforms of the mouse ELP gene.
Ninomiya Y, Okada M, Kotomura N, Suzuki K, Tsukiyama T, Niwa O.
Journal of Biochemistry 1995 Aug; 118(2):380.
Application：WB-Ce, WB-Tr, Human, Mammalian cells.
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hELP3 subunit of the Elongator complex regulates the transcription of HSP70 gene in human cells.
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