HDAC9 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of HDAC9.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human HDAC9.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
Immunoprecipitation (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the HDAC9 polyclonal antibody (Cat # PAB2339) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.Immunoprecipitation
Both anti-HDAC9 N-term (Cat # PAB2238) and C-term (Cat # PAB2339) polyclonal antibody were tested by WB and IP-WB using HeLa and HeLa-HDAC9 transfected cells.
Top figure shows both polyclonal antibody specifically detect HDAC9 in HeLa-HDAC9 transfected cell but not HeLa alone.
Bottom figure shows that both polyclonal antibody can immunoprecipitate (IP) HDAC9 from HeLa-HDAC9 tranfected cells.
(Data kindly provided by Dr. Zhigang Yuan, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL) . -
Gene Info — HDAC9
Entrez GeneID
9734Protein Accession#
Q9UKV0Gene Name
HDAC9
Gene Alias
DKFZp779K1053, HD7, HDAC, HDAC7, HDAC7B, HDAC9B, HDAC9FL, HDRP, KIAA0744, MITR
Gene Description
histone deacetylase 9
Omim ID
606543Gene Ontology
HyperlinkGene Summary
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. Multiple alternatively spliced transcripts have been described for this gene but the full-length nature of some of them has not been determined. [provided by RefSeq
Other Designations
MEF-2 interacting transcription repressor (MITR) protein|histone deacetylase 4/5-related protein|histone deacetylase 7|histone deacetylase 7B
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Interactome
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Disease
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Publication Reference
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Molecular characterization of a familial translocation implicates disruption of HDAC9 and possible position effect on TGFbeta2 in the pathogenesis of Peters' anomaly.
David D, Cardoso J, Marques B, Marques R, Silva ED, Santos H, Boavida MG.
Genomics 2003 May; 81(5):489.
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The histone deacetylase 9 gene encodes multiple protein isoforms.
Petrie K, Guidez F, Howell L, Healy L, Waxman S, Greaves M, Zelent A.
The Journal of Biological Chemistry 2003 May; 278(18):16059.
Application:IF, WB-Tr, Human, Monkey, COS-7, HEK 293T cells.
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Chromosomal organization and localization of the human histone deacetylase 9 gene (HDAC9).
Mahlknecht U, Schnittger S, Will J, Cicek N, Hoelzer D.
Biochemical and Biophysical Research Communications 2002 Apr; 293(1):182.
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Molecular characterization of a familial translocation implicates disruption of HDAC9 and possible position effect on TGFbeta2 in the pathogenesis of Peters' anomaly.
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