HDAC8 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HDAC8 polyclonal antibody (Cat # PAB2336) in mouse NIH/3T3 cell lysate. HDAC8 (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the HDAC8 polyclonal antibody (Cat # PAB2336) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of HDAC8.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human HDAC8.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HDAC8 polyclonal antibody (Cat # PAB2336) in mouse NIH/3T3 cell lysate. HDAC8 (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the HDAC8 polyclonal antibody (Cat # PAB2336) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — HDAC8
Entrez GeneID
55869Protein Accession#
Q9BY41Gene Name
HDAC8
Gene Alias
HDACL1, RPD3
Gene Description
histone deacetylase 8
Omim ID
300269Gene Ontology
HyperlinkGene Summary
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. [provided by RefSeq
Other Designations
histone deacetylase-like 1
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Interactome
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Disease
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Publication Reference
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Cloning and characterization of human histone deacetylase 8.
Van den Wyngaert I, de Vries W, Kremer A, Neefs J, Verhasselt P, Luyten WH, Kass SU.
FEBS Letters 2000 Jul; 478(1-2):77.
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Cloning and characterization of a novel human class I histone deacetylase that functions as a transcription repressor.
Hu E, Chen Z, Fredrickson T, Zhu Y, Kirkpatrick R, Zhang GF, Johanson K, Sung CM, Liu R, Winkler J.
The Journal of Biological Chemistry 2000 May; 275(20):15254.
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Characterization of a highly complex region in Xq13 and mapping of three isodicentric breakpoints associated with preleukemia.
McDonell N, Ramser J, Francis F, Vinet MC, Rider S, Sudbrak R, Riesselman L, Yaspo ML, Reinhardt R, Monaco AP, Ross F, Kahn A, Kearney L, Buckle V, Chelly J.
Genomics 2000 Mar; 64(3):221.
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Cloning and characterization of human histone deacetylase 8.
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