TCHP polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant TCHP.
Immunogen
Recombinant protein corresponding to amino acids of human TCHP.
Sequence
MEAFRQKAELGRFLRHQYNAQLSRRTQQIQEELEADRRILQALLEKEDESQRLHLARREQVMADVAWMKQAIEEQ
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:1000-1:2500)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with TCHP polyclonal antibody (Cat # PAB23320) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human liver with TCHP polyclonal antibody (Cat # PAB23320) shows distinct membranous positivity in hepatocytes at 1:1000-1:2500 dilution.Immunofluorescence
Immunofluorescent staining of human cell line A-431 with TCHP polyclonal antibody (Cat # PAB23320) at 1-4 ug/mL dilution shows positivity in plasma membrane, cytoplasm and centrosome. -
Gene Info — TCHP
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Interactome
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Publication Reference
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Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.
Tu HQ, Qin XH, Liu ZB, Song ZQ, Hu HB, Zhang YC, Chang Y, Wu M, Huang Y, Bai YF, Wang G, Han QY, Li AL, Zhou T, Liu F, Zhang XM, Li HY.
Nature Communications 2018 Dec; 9(1):5277.
Application:WB-Tr, Human, HEK 293T cells.
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Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.
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