SGK3 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SGK3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human SGK3.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of SGK3 polyclonal antibody (Cat # PAB2320) in A-375 cell lysate. SGK3 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the SGK3 polyclonal antibody (Cat # PAB2320) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — SGK3
Entrez GeneID
23678Protein Accession#
Q96BR1Gene Name
SGK3
Gene Alias
CISK, DKFZp781N0293, SGK2, SGKL
Gene Description
serum/glucocorticoid regulated kinase family, member 3
Omim ID
607591Gene Ontology
HyperlinkGene Summary
This gene is a member of the Ser/Thr protein kinase family and encodes a phosphoprotein with a PX (phox homology) domain. The protein phosphorylates several target proteins and has a role in neutral amino acid transport and activation of potassium and chloride channels. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
cytokine-independent survival kinase|serum/glucocorticoid regulated kinase 3|serum/glucocorticoid regulated kinase-like
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Interactome
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Publication Reference
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The critical roles of serum/glucocorticoid-regulated kinase 3 (SGK3) in the hair follicle morphogenesis and homeostasis: the allelic difference provides novel insights into hair follicle biology.
Okada T, Ishii Y, Masujin K, Yasoshima A, Matsuda J, Ogura A, Nakayama H, Kunieda T, Doi K.
The American Journal of Pathology 2006 Apr; 168(4):1119.
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The critical roles of serum/glucocorticoid-regulated kinase 3 (SGK3) in the hair follicle morphogenesis and homeostasis: the allelic difference provides novel insights into hair follicle biology.
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