C10orf32 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant C10orf32.
Immunogen
Recombinant protein corresponding to amino acids of human C10orf32.
Sequence
FGQSVKGLLTEKVTTCGTDVIALTKQVLKGSRSSELLGQAARNMVLQEDAILHSEDSLRKMAIITTHLQYQQEAIQKNVEQSSDLQDQLNHLLK
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:500-1:1000)
Western Blot (1:250-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with C10orf32 polyclonal antibody (Cat # PAB23142) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human colon with C10orf32 polyclonal antibody (Cat # PAB23142) shows moderate cytoplasmic and nuclear positivity in glandular cells at 1:500-1:1000 dilution. -
Gene Info — C10orf32
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Interactome
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Publication Reference
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Inhibition of endolysosome fusion increases exosome secretion.
Ganesh Vilas Shelke, Chad D Williamson, Michal Jarnik, Juan S Bonifacino.
The Journal of Cell Biology 2023 Jun; 222(6):e202209084.
Application:WB-Ce, Human, HeLA cells.
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Synaptic Vesicle Precursors and Lysosomes Are Transported by Different Mechanisms in the Axon of Mammalian Neurons.
Raffaella De Pace, Dylan J Britt, Jeffrey Mercurio, Arianne M Foster, Lucas Djavaherian, Victoria Hoffmann, Daniel Abebe, Juan S Bonifacino.
Cell Reports 2020 Jun; 31(11):107775.
Application:WB-Tr, Mouse, MEFs.
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Inhibition of endolysosome fusion increases exosome secretion.
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