RGNEF polyclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human duodenum with RGNEF polyclonal antibody (Cat # PAB23138) shows strong cytoplasmic positivity in glandular cells.
Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with RGNEF polyclonal antibody (Cat # PAB23138) at 1-4 ug/mL dilution shows positivity in cytoplasm and cytoskeleton (microtubules).
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant RGNEF.
Immunogen
Recombinant protein corresponding to amino acids of human RGNEF.
Sequence
GTAHTEAQQSFMSPSSSCASNLNLSFGWHGFEKEQSHLKKRSSSLDALDADSEGEGHSEPSHICYTPGSQSSSRTGIPSGDELDSFETNTEPDFNI
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:200-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human duodenum with RGNEF polyclonal antibody (Cat # PAB23138) shows strong cytoplasmic positivity in glandular cells.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with RGNEF polyclonal antibody (Cat # PAB23138) at 1-4 ug/mL dilution shows positivity in cytoplasm and cytoskeleton (microtubules). -
Gene Info — RGNEF
Entrez GeneID
64283Protein Accession#
Q8N1W1Gene Name
RGNEF
Gene Alias
DKFZp686P12164, FLJ21817, KIAA1998
Gene Description
Rho-guanine nucleotide exchange factor
Gene Ontology
HyperlinkGene Summary
RGNEF is a member of the Rho guanine nucleotide exchange factor (GEF) family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP.[supplied by OMIM
Other Designations
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Interactome
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Disease
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