GEMIN5 polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with GEMIN5 polyclonal antibody (Cat # PAB23104) at 1:250-1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cerebellum with GEMIN5 polyclonal antibody (Cat # PAB23104) shows strong cytoplasmic and nucleolar positivity in purkinje cells at 1:200-1:500 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line A-431 with GEMIN5 polyclonal antibody (Cat # PAB23104) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli and cytoplasm.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant GEMIN5.
Immunogen
Recombinant protein corresponding to amino acids of human GEMIN5.
Sequence
YDSGSFTIMQEVYSAFLPDGCDHLRDKLGDHQSPATPAFKSLEAFFLYGRLYEFWWSLSRPCPNSSVWVRAGHRTLSVEPSQQLDTASTEETDPET
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:200-1:500)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with GEMIN5 polyclonal antibody (Cat # PAB23104) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cerebellum with GEMIN5 polyclonal antibody (Cat # PAB23104) shows strong cytoplasmic and nucleolar positivity in purkinje cells at 1:200-1:500 dilution.Immunofluorescence
Immunofluorescent staining of human cell line A-431 with GEMIN5 polyclonal antibody (Cat # PAB23104) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli and cytoplasm. -
Gene Info — GEMIN5
Entrez GeneID
25929Protein Accession#
Q8TEQ6Gene Name
GEMIN5
Gene Alias
DKFZp586M1824, MGC142174
Gene Description
gem (nuclear organelle) associated protein 5
Omim ID
607005Gene Ontology
HyperlinkGene Summary
GEMIN5 is part of a large macromolecular complex localized to both the cytoplasm and the nucleus that plays a role in the cytoplasmic assembly of small nuclear ribonucleoproteins (snRNPs). Other members of this complex include SMN (MIM 600354), GEMIN2 (SIP1; MIM 602595), GEMIN3 (DDX20; MIM 606168), and GEMIN4 (MIM 606969).[supplied by OMIM
Other Designations
gemin 5
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