PKN3 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PKN3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human PKN3.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of PKN3 polyclonal antibody (Cat # PAB2307) in HeLa cell lysate. PKN3 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with PKN3 polyclonal antibody (Cat # PAB2307) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. -
Gene Info — PKN3
Entrez GeneID
29941Protein Accession#
Q9UM03;NP_037487Gene Name
PKN3
Gene Alias
RP11-545E17.1
Gene Description
protein kinase N3
Omim ID
610714Gene Ontology
HyperlinkOther Designations
OTTHUMP00000022293|protein kinase PKNbeta
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Interactome
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Publication Reference
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Identification and characterization of PKNbeta, a novel isoform of protein kinase PKN: expression and arachidonic acid dependency are different from those of PKNalpha.
Oishi K, Mukai H, Shibata H, Takahashi M, Ona Y.
Biochemical and Biophysical Research Communications 1999 Aug; 261(3):808.
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Identification and characterization of PKNbeta, a novel isoform of protein kinase PKN: expression and arachidonic acid dependency are different from those of PKNalpha.
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