STT3B polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant STT3B.
Immunogen
Recombinant protein corresponding to amino acids of human STT3B.
Sequence
NPPVEDSSDEDDKRNQGNLYDKAGKVRKHATEQEKTEEGLGP
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:500-1:1000)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human testis with STT3B polyclonal antibody (Cat # PAB23044) shows strong cytoplasmic positivity in spermatogonia.Immunofluorescence
Immunofluorescent staining of human cell line A-431 with STT3B polyclonal antibody (Cat # PAB23044) at 1-4 ug/mL dilution shows positivity in endoplasmic reticulum. -
Gene Info — STT3B
Entrez GeneID
201595Protein Accession#
Q8TCJ2Gene Name
STT3B
Gene Alias
FLJ90106, SIMP, STT3-B
Gene Description
STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae)
Omim ID
608605Gene Ontology
HyperlinkGene Summary
The SIMP protein contains a highly immunogenic minor histocompatibility antigen epitope of 9 amino acids, B6(dom1). Like ITM1 (MIM 601134), SIMP is homologous to yeast STT3, an oligosaccharyltransferase essential for cell proliferation (McBride et al., 2002 [PubMed 12439619]).[supplied by OMIM
Other Designations
OTTHUMP00000161258|homolog of yeast STT3|source of immunodominant MHC associated peptides|source of immunodominant MHC-associated peptides
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