RBM15B polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant RBM15B.
Immunogen
Recombinant protein corresponding to amino acids of human RBM15B.
Sequence
GKKARDSERNHRTTEAEPKPLEEPKHETKKLKNLSEYAQTLQLGWNGLLVLKNSCFPTSMHILEGDQGVISSLLKDHTSG
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:500-1:1000)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with RBM15B polyclonal antibody (Cat # PAB23043) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human rectum with RBM15B polyclonal antibody (Cat # PAB23043) shows strong cytoplasmic positivity in endocrine cells at 1:500-1:1000 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with RBM15B polyclonal antibody (Cat # PAB23043) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli. -
Gene Info — RBM15B
Entrez GeneID
29890Protein Accession#
Q8NDT2Gene Name
RBM15B
Gene Alias
HUMAGCGB, OTT3
Gene Description
RNA binding motif protein 15B
Gene Ontology
HyperlinkGene Summary
Members of the SPEN (Split-end) family of proteins, including RBM15B, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components (Hiriart et al., 2005 [PubMed 16129689]).[supplied by OMIM
Other Designations
chromosome 3p21.1 gene sequence|one twenty two protein 3
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