PAK6 polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot analysis of PAK6 polyclonal antibody (Cat # PAB2303) in lysates from transiently transfected COS-7 cells.
Lane 1 : negative control.
Lane 2 : PAK1-expressing cells.
Lane 3 : PAK2-expressing cells
Lane 4 : PAK4-expressing cells.
Lane 5 : PAK5-expressing cells.
Lane 6 : PAK6-expressing cells.
PAK6 (arrow) was detected using purified polyclonal antibody. Data is kindly provided by Drs. Z.M. Jaffer and J. Chernoff from the Fox Chase Cancer Center (Philadelphia, PA) .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PAK6.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 131-146 of human PAK6.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunoprecipitation (1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of PAK6 polyclonal antibody (Cat # PAB2303) in lysates from transiently transfected COS-7 cells.
Lane 1 : negative control.
Lane 2 : PAK1-expressing cells.
Lane 3 : PAK2-expressing cells
Lane 4 : PAK4-expressing cells.
Lane 5 : PAK5-expressing cells.
Lane 6 : PAK6-expressing cells.
PAK6 (arrow) was detected using purified polyclonal antibody. Data is kindly provided by Drs. Z.M. Jaffer and J. Chernoff from the Fox Chase Cancer Center (Philadelphia, PA) .Western Blot
Immunoprecipitation
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Gene Info — PAK6
Entrez GeneID
56924Protein Accession#
NP_064553Gene Name
PAK6
Gene Alias
PAK5
Gene Description
p21 protein (Cdc42/Rac)-activated kinase 6
Omim ID
608110Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the p21-activated kinase (PAK) family. The proteins of this family are Rac/Cdc42-associated Ste20-like Ser/Thr protein kinases, characterized by a highly conserved amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. PAK kinases are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. The protein encoded by this gene was found to interact with androgen receptor (AR), which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development. This gene was found to be highly expressed in testis and prostate tissues and the encoded protein was shown to cotranslocate into the nucleus with AR in response to androgen. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
p21(CDKN1A)-activated kinase 6|p21-activated kinase 6|p21-activated protein kinase 6
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Interactome
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Pathway
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Disease
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Publication Reference
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Identification of an autoinhibitory domain of p21-activated protein kinase 5.
Ching YP, Leong VY, Wong CM, Kung HF.
The Journal of Biological Chemistry 2003 Sep; 278(36):33621.
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Cloning and characterization of PAK5, a novel member of mammalian p21-activated kinase-II subfamily that is predominantly expressed in brain.
Pandey A, Dan I, Kristiansen TZ, Watanabe NM, Voldby J, Kajikawa E, Khosravi-Far R, Blagoev B, Mann M.
Oncogene 2002 May; 21(24):3939.
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Androgen receptor specifically interacts with a novel p21-activated kinase, PAK6.
Yang F, Li X, Sharma M, Zarnegar M, Lim B, Sun Z.
The Journal of Biological Chemistry 2001 May; 276(18):15345.
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Identification of an autoinhibitory domain of p21-activated protein kinase 5.
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