Western blot analysis of PAK7 polyclonal antibody (Cat # PAB2302) in lysates from transiently transfected COS-7 cells. Lane 1 : negative control. Lane 2 : PAK1-expressing cells. Lane 3 : PAK2-expressing cells. Lane 4 : PAK4-expressing cells. Lane 5 : PAK5-expressing cells. Lane 6 : PAK6-expressing cells. PAK5 (arrow) was detected using purified polyclonal antibody. Data is kindly provided by Drs. Z.M. Jaffer and J. Chernoff from the Fox Chase Cancer Center (Philadelphia, PA) .
Formalin-fixed and paraffin-embedded human brain tissue reacted with PAK7 polyclonal antibody (Cat # PAB2302) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
The protein encoded by this gene is a member of the PAK family of Ser/Thr protein kinases. PAK family members are known to be effectors of Rac/Cdc42 GTPases, which have been implicated in the regulation of cytoskeletal dynamics, proliferation, and cell survival signaling. This kinase contains a CDC42/Rac1 interactive binding (CRIB) motif, and has been shown to bind CDC42 in the presence of GTP. This kinase is predominantly expressed in brain. It is capable of promoting neurite outgrowth, and thus may play a role in neurite development. This kinase is associated with microtubule networks and induces microtubule stabilization. The subcellular localization of this kinase is tightly regulated during cell cycle progression. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq