Western blot analysis of PAK3 (arrow) using PAK3 polyclonal antibody (Cat # PAB2300). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PAK3 gene (Lane 2).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the PAK3 polyclonal antibody (Cat # PAB2300) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. The protein encoded by this gene forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1 proteins which then catalyzes a variety of targets. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in this gene are the cause of non-syndromic mental retardation X-linked type 30 (MRX30), also called X-linked mental retardation type 47 (MRX47). Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
OTTHUMP00000023855,OTTHUMP00000062894,beta-PAK,oligophrenin-3,p21 (CDKN1A)-activated kinase 3,p21-activated kinase 3,p21-activated kinase-3,serine/threonine-protein kinase PAK 3