ANKRD23 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant ANKRD23.
Immunogen
Recombinant protein corresponding to amino acids of human ANKRD23.
Sequence
KRLRHRVPPRKPEPLVKPQSQAQVEPVGLEMFLKAAAENQEYLIDKYLTDGGDPNAHDKLHRTALHWAC
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:200-1:500)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with ANKRD23 polyclonal antibody (Cat # PAB22965) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human smooth muscle with ANKRD23 polyclonal antibody (Cat # PAB22965) shows strong cytoplasmic positivity in smooth muscle cells at 1:200-1:500 dilution.Immunofluorescence
Immunofluorescent staining of human cell line A-431 with ANKRD23 polyclonal antibody (Cat # PAB22965) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli and cytoplasm. -
Gene Info — ANKRD23
Entrez GeneID
200539Protein Accession#
Q86SG2Gene Name
ANKRD23
Gene Alias
DARP, FLJ32449, MARP3, MGC129593
Gene Description
ankyrin repeat domain 23
Omim ID
610736Gene Ontology
HyperlinkGene Summary
This gene is a member of the muscle ankyrin repeat protein (MARP) family and encodes a protein with four tandem ankyrin-like repeats. The protein is localized to the nucleus, functioning as a transcriptional regulator. Expression of this protein is induced during recovery following starvation. [provided by RefSeq
Other Designations
diabetes related ankyrin repeat protein|muscle ankyrin repeat protein 3
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