DHX36 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant DHX36.
Immunogen
Recombinant protein corresponding to amino acids of human DHX36.
Sequence
ERREEQIVQLLNSVQAKNDKESEAQISWFAPEDHGYGTEVSTKNTPCSENKLDIQEKKLINQEKKMFRIRNRSYIDRDSEYLLQENEPDGT
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:20-1:50)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with DHX36 polyclonal antibody (Cat # PAB22871) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human testis with DHX36 polyclonal antibody (Cat # PAB22871) shows strong nuclear and cytoplasmic positivity in cells of seminiferus ducts at 1:20-1:50 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with DHX36 polyclonal antibody (Cat # PAB22871) at 1-4 ug/mL dilution shows positivity in cytoplasm. -
Gene Info — DHX36
Entrez GeneID
170506Protein Accession#
Q9H2U1Gene Name
DHX36
Gene Alias
DDX36, G4R1, KIAA1488, MLEL1, RHAU
Gene Description
DEAH (Asp-Glu-Ala-His) box polypeptide 36
Gene Ontology
HyperlinkGene Summary
This gene is a member of the DEAH-box family of RNA-dependent NTPases which are named after the conserved amino acid sequence Asp-Glu-Ala-His in motif II. The protein encoded by this gene has been shown to enhance the deadenylation and decay of mRNAs with 3'-UTR AU-rich elements (ARE-mRNA). The protein has also been shown to resolve into single strands the highly stable tetramolecular DNA configuration (G4) that can form spontaneously in guanine-rich regions of DNA. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 36|G4 resolvase-1|RNA helicase associated with AU-rich element
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Interactome
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Disease
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