MAP2K4 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MAP2K4.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to residues surrounding S257of human MAP2K4.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of MAP2K4 (arrow) using rabbit MAP2K4 polyclonal antibody (Cat # PAB2287). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K4 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with MAP2K4 polyclonal antibody (Cat # PAB2287) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. -
Gene Info — MAP2K4
Entrez GeneID
6416Protein Accession#
NP_003001;P45985Gene Name
MAP2K4
Gene Alias
JNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SEK1, SERK1
Gene Description
mitogen-activated protein kinase kinase 4
Omim ID
601335Gene Ontology
HyperlinkGene Summary
This gene encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver. [provided by RefSeq
Other Designations
JNK activating kinase 1|JNK-activated kinase 1|MAP kinase kinase 4|MAPK/ERK kinase 4|SAPK/ERK kinase 1|c-Jun N-terminal kinase kinase 1|dual specificity mitogen-activated protein kinase kinase 4
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Interactome
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Pathway
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Publication Reference
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Mitogen-activated protein kinase kinase 4/c-Jun NH2-terminal kinase kinase 1 protein expression is subject to translational regulation in prostate cancer cell lines.
Robinson VL, Shalhav O, Otto K, Kawai T, Gorospe M, Rinker-Schaeffer CW.
Molecular Cancer Research: MCR 2008 Mar; 6(3):501.
Application:WB-Ce, Human, LNCap, PC3, SKOV3ip.1 cells.
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RIP1-mediated AIP1 phosphorylation at a 14-3-3-binding site is critical for tumor necrosis factor-induced ASK1-JNK/p38 activation.
Zhang H, Zhang H, Lin Y, Li J, Pober JS, Min W.
The Journal of Biological Chemistry 2007 May; 282(20):14788.
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Activation of MEK-1 and SEK-1 by Tpl-2 proto-oncoprotein, a novel MAP kinase kinase kinase.
Salmeron A, Ahmad TB, Carlile GW, Pappin D, Narsimhan RP, Ley SC.
The EMBO Journal 1996 Feb; 15(4):817.
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Mitogen-activated protein kinase kinase 4/c-Jun NH2-terminal kinase kinase 1 protein expression is subject to translational regulation in prostate cancer cell lines.
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