SF3B14 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant SF3B14.
Immunogen
Recombinant protein corresponding to amino acids of human SF3B14.
Sequence
YEDIFDAKNACDHLSGFNVCNRYLVVLYYNANRAFQKMDTKKKEEQLKLLKEKYGINTDPP
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:250-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with SF3B14 polyclonal antibody (Cat # PAB22831) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cerebral cortex with SF3B14 polyclonal antibody (Cat # PAB22831) shows strong nuclear positivity in neuronal cells at 1:50-1:200 dilution. -
Gene Info — SF3B14
Entrez GeneID
51639Protein Accession#
Q9Y3B4Gene Name
SF3B14
Gene Alias
CGI-110, HSPC175, Ht006, P14, SAP14, SF3B14a
Gene Description
splicing factor 3B, 14 kDa subunit
Omim ID
607835Gene Ontology
HyperlinkGene Summary
This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site. [provided by RefSeq
Other Designations
pre-mRNA branch site protein p14|spliceosome-associated protein, 14 kDa subunit
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Interactome
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Disease
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