MAP3K8 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of MAP3K8 polyclonal antibody (Cat # PAB2282) in A-375 cell lysate. MAP3K8 (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the MAP3K8 polyclonal antibody (Cat # PAB2282) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MAP3K8.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human MAP3K8.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of MAP3K8 polyclonal antibody (Cat # PAB2282) in A-375 cell lysate. MAP3K8 (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the MAP3K8 polyclonal antibody (Cat # PAB2282) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — MAP3K8
Entrez GeneID
1326Protein Accession#
P41279Gene Name
MAP3K8
Gene Alias
COT, EST, ESTF, FLJ10486, TPL2, Tpl-2, c-COT
Gene Description
mitogen-activated protein kinase kinase kinase 8
Gene Ontology
HyperlinkGene Summary
This gene was identified by its oncogenic transforming activity in cells. The encoded protein is a member of the serine/threonine protein kinase family. This kinase can activate both the MAP kinase and JNK kinase pathways. This kinase was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This kinase was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. Studies of a similar gene in rat suggested the direct involvement of this kinase in the proteolysis of NF-kappaB1,p105 (NFKB1). This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. [provided by RefSeq
Other Designations
Cancer Osaka thyroid oncogene|Ewing sarcoma transformant|OTTHUMP00000019392|OTTHUMP00000019393|cot (cancer Osaka thyroid) oncogene|proto-oncogene serine/threoine protein kinase|tumor progression locus-2
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
COT kinase proto-oncogene expression in T cells: implication of the JNK/SAPK signal transduction pathway in COT promoter activation.
Sanchez-Gongora E, Lisbona C, de Gregorio R, Ballester A, Calvo V, Perez-Jurado L, Alemany S.
The Journal of Biological Chemistry 2000 Oct; 275(40):31379.
-
The human cot proto-oncogene encodes two protein serine/threonine kinases with different transforming activities by alternative initiation of translation.
Aoki M, Hamada F, Sugimoto T, Sumida S, Akiyama T, Toyoshima K.
The Journal of Biological Chemistry 1993 Oct; 298(30):22723.
-
COT kinase proto-oncogene expression in T cells: implication of the JNK/SAPK signal transduction pathway in COT promoter activation.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com