DPH3 polyclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human spleen with DPH3 polyclonal antibody (Cat # PAB22674) shows distinct cytoplasmic positivity in cells in red pulp.
Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with DPH3 polyclonal antibody (Cat # PAB22674) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli and cytoplasm.
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Specifications
Product Description
Rabbit polyclonal antibody raised against recombinant DPH3.
Immunogen
Recombinant protein corresponding to amino acids of human DPH3.
Sequence
EDFQYDEDSETYFYPCPCGDNFSITKEDLENGEDVATCPSCSLIIKVIYDKDQFVCGETVPAPSANKELVK
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human spleen with DPH3 polyclonal antibody (Cat # PAB22674) shows distinct cytoplasmic positivity in cells in red pulp.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with DPH3 polyclonal antibody (Cat # PAB22674) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli and cytoplasm. -
Gene Info — DPH3
Entrez GeneID
285381Protein Accession#
Q96FX2Gene Name
DPH3
Gene Alias
DELGIP, DELGIP1, DESR1, DPH3A, KTI11, MGC20197, ZCSL2
Gene Description
DPH3, KTI11 homolog (S. cerevisiae)
Omim ID
608959Gene Ontology
HyperlinkGene Summary
This gene encodes a CSL zinc finger-containing protein that is required for dipthamide biosynthesis. The encoded protein is necessary for the initial step in the modification of a histidine residue in elongation factor-2 to diphthamide. This modified residue is a target for ADP ribosylation by the bacterial toxins diphtheria toxin and Pseudomonas exotoxin A. Alternative splicing results in multiple transcript variants that encode the same isoform. [provided by RefSeq
Other Designations
DPH3 homolog (KTI11, S. cerevisiae)|DPH3A, KTI11 homolog A|deafness locus putative guanine nucleotide exchange factor interacting protein 1|zinc finger, CSL domain containing 2|zinc finger, CSL-type containing 2
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