PPCS polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with PPCS polyclonal antibody (Cat # PAB22546) at 1:250-1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human kidney with PPCS polyclonal antibody (Cat # PAB22546) shows strong cytoplasmic and nuclear positivity in cells in tubules at 1:200-1:500 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant PPCS.
Immunogen
Recombinant protein corresponding to amino acids of human PPCS.
Sequence
VLFLYRARSAFPYAHRFPPQTWLSALRPSGPALSGLLSLEAEENALPGFAEALRSYQEAAAAGTFLAVEFTTLADY
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:200-1:500)
Western Blot (1:250-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with PPCS polyclonal antibody (Cat # PAB22546) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human kidney with PPCS polyclonal antibody (Cat # PAB22546) shows strong cytoplasmic and nuclear positivity in cells in tubules at 1:200-1:500 dilution. -
Gene Info — PPCS
Entrez GeneID
79717Protein Accession#
Q9HAB8Gene Name
PPCS
Gene Alias
FLJ11838, MGC117357, MGC138220
Gene Description
phosphopantothenoylcysteine synthetase
Omim ID
609853Gene Ontology
HyperlinkGene Summary
Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCS (EC 6.3.2.5), one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM
Other Designations
OTTHUMP00000008431|OTTHUMP00000008433
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Interactome
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Pathway
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