PISD polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant PISD.
Immunogen
Recombinant protein corresponding to amino acids of human PISD.
Sequence
YKSVPTRLLSRAWGRLNQVELPHWLRRPVYSLYIWTFGVNMKEAAVEDLHHYRNLSEFFRRKLKPQARPVCGLHSISPSD
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:200-1:500)
Western Blot (1:250-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with PISD polyclonal antibody (Cat # PAB22508) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human tonsil with PISD polyclonal antibody (Cat # PAB22508) shows strong cytoplasmic positivity in reaction center cells at 1:200-1:500 dilution. -
Gene Info — PISD
Entrez GeneID
23761Protein Accession#
Q9UG56Gene Name
PISD
Gene Alias
DJ858B16, DKFZp566G2246, PSDC, PSSC, dJ858B16.2
Gene Description
phosphatidylserine decarboxylase
Gene Ontology
HyperlinkGene Summary
Phosphatidylserine decarboxylases (PSDs; EC 4.1.1.65) catalyze the formation of phosphatidylethanolamine (PE) by decarboxylation of phosphatidylserine (PS). Type I PSDs, such as PISD, are targeted to the inner mitochondrial membrane by an N-terminal targeting sequence. PISD also contains a conserved LGST motif that functions as an autocatalytic cleavage site where the proenzyme is split into mature alpha and beta subunits (Schuiki and Daum, 2009 [PubMed 19165886]).[supplied by OMIM
Other Designations
OTTHUMP00000063301
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