TYW3 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant TYW3.
Immunogen
Recombinant protein corresponding to amino acids of human TYW3.
Sequence
EFRKWKAQCLSKADLSRKGSVDEDVVELVQFLNMRDQFFTTSSCAGRILLLDRGINGFEVQKQNCCWLLVTHKLCVKDDVIV
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:500-1:1000)
Western Blot (1:250-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with TYW3 polyclonal antibody (Cat # PAB22321) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human testis with TYW3 polyclonal antibody (Cat # PAB22321) shows moderate cytoplasmic positivity in seminiferus duct cells at 1:500-1:1000 dilution. -
Gene Info — TYW3
Entrez GeneID
127253Protein Accession#
Q6IPR3Gene Name
TYW3
Gene Alias
C1orf171, FLJ40918
Gene Description
tRNA-yW synthesizing protein 3 homolog (S. cerevisiae)
Omim ID
611245Gene Ontology
HyperlinkGene Summary
Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW3 is the human homolog of a yeast gene essential for yW synthesis (Noma and Suzuki, 2006).[supplied by OMIM
Other Designations
OTTHUMP00000011215|tRNA-yW synthesizing protein 3 homolog
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