CCT4 polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with CCT4 polyclonal antibody (Cat # PAB22272) at 1:250-1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human testis with CCT4 polyclonal antibody (Cat # PAB22272) shows strong cytoplasmic positivity in cells in seminiferus ducts at 1:50-1:200 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with CCT4 polyclonal antibody (Cat # PAB22272) at 1-4 ug/mL dilution shows positivity in cytoplasm.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant CCT4.
Immunogen
Recombinant protein corresponding to amino acids of human CCT4.
Sequence
GSNKLVIEEAERSIHDALCVIRCLVKKRALIAGGGAPEIELALRLTEYSRTLSGMESYCVRAFADAMEVIPSTLAENAGLNPISTVTELRNRHAQGEKTAGINVRKGGISNILEELVVQPLLVSVSALTLATETVRSILK
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with CCT4 polyclonal antibody (Cat # PAB22272) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human testis with CCT4 polyclonal antibody (Cat # PAB22272) shows strong cytoplasmic positivity in cells in seminiferus ducts at 1:50-1:200 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with CCT4 polyclonal antibody (Cat # PAB22272) at 1-4 ug/mL dilution shows positivity in cytoplasm. -
Gene Info — CCT4
Entrez GeneID
10575Protein Accession#
P50991Gene Name
CCT4
Gene Alias
CCT-DELTA, Cctd, MGC126164, MGC126165, SRB
Gene Description
chaperonin containing TCP1, subunit 4 (delta)
Omim ID
605142Gene Ontology
HyperlinkGene Summary
The chaperonin containing TCP1 (MIM 186980) complex (CCT), also called the TCP1 ring complex, consists of 2 back-to-back rings, each containing 8 unique but homologous subunits, such as CCT4. CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP-driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin (see MIM 102560) and tubulin (see MIM 191130), as well as alpha-transducin (MIM 139330) (Won et al., 1998 [PubMed 9819444]).[supplied by OMIM
Other Designations
chaperonin containing t-complex polypeptide 1, delta subunit
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