SMYD2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SMYD2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human SMYD2.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of SYMD2 (arrow) using rabbit SMYD2 polyclonal antibody (Cat # PAB2223). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SYMD2 gene (Lane 2) (Origene Technologies). -
Gene Info — SMYD2
Entrez GeneID
56950Protein Accession#
NP_064582;Q9NRG4Gene Name
SMYD2
Gene Alias
HSKM-B, KMT3C, MGC119305, ZMYND14
Gene Description
SET and MYND domain containing 2
Omim ID
610663Gene Ontology
HyperlinkGene Summary
SET domain-containing proteins, such as SMYD2, catalyze lysine methylation (Brown et al., 2006 [PubMed 16805913]).[supplied by OMIM
Other Designations
OTTHUMP00000035134|zinc finger, MYND domain containing 14
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Interactome
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Disease
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Publication Reference
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The tale of two domains: proteomics and genomics analysis of SMYD2, a new histone methyltransferase.
Abu-Farha M, Lambert JP, Al-Madhoun AS, Elisma F, Skerjanc IS, Figeys D.
Molecular & Cellular Proteomics 2008 Mar; 7(3):560.
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Large-scale mapping of human protein-protein interactions by mass spectrometry.
Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D.
Molecular Systems Biology 2007 Mar; 3:89.
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The tale of two domains: proteomics and genomics analysis of SMYD2, a new histone methyltransferase.
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