MBD3 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of MBD3 (arrow) using rabbit MBD3 polyclonal antibody (Cat # PAB2214). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MBD3 gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the MBD3 polyclonal antibody (Cat # PAB2214) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MBD3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to residues surrounding E279 of human MBD3.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Transfected lysate)
Western blot analysis of MBD3 (arrow) using rabbit MBD3 polyclonal antibody (Cat # PAB2214). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MBD3 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the MBD3 polyclonal antibody (Cat # PAB2214) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — MBD3
Entrez GeneID
53615Protein Accession#
O95983Gene Name
MBD3
Gene Alias
-
Gene Description
methyl-CpG binding domain protein 3
Omim ID
603573Gene Ontology
HyperlinkGene Summary
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex. [provided by RefSeq
Other Designations
-
-
Interactome
-
Publication Reference
-
Biphasic kinetics of the human DNA repair protein MED1 (MBD4), a mismatch-specific DNA N-glycosylase.
Petronzelli F, Riccio A, Markham GD, Seeholzer SH, Stoerker J, Genuardi M, Yeung AT, Matsumoto Y, Bellacosa A.
The Journal of Biological Chemistry 2000 Oct; 275(42):32422.
-
A mammalian protein with specific demethylase activity for mCpG DNA.
Bhattacharya SK, Ramchandani S, Cervoni N, Szyf M.
Nature 1999 Feb; 397(6720):579.
-
Identification and characterization of a family of mammalian methyl-CpG binding proteins.
Hendrich B, Bird A.
Molecular and Cellular Biology 1998 Nov; 18(11):6538.
Application：Band shift assay, Recombinant protein.
-
Biphasic kinetics of the human DNA repair protein MED1 (MBD4), a mismatch-specific DNA N-glycosylase.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com