PARP10 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant PARP10.
Immunogen
Recombinant protein corresponding to amino acids of human PARP10.
Sequence
PHYDILEPEELAENTSGGDHPSTQGPRATKHALLRTGGLVTALQGAGTVTMGSGEEPGQSGTSLRTGPMVQGRGIMTTGSGQEPGQSGTSL
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:20-1:50)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with PARP10 polyclonal antibody (Cat # PAB22109) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human thyroid gland with PARP10 polyclonal antibody (Cat # PAB22109) shows distinct cytoplasmic positivity in glandular cells at 1:20-1:50 dilution.Immunofluorescence
Immunofluorescent staining of human cell line A-431 with PARP10 polyclonal antibody (Cat # PAB22109) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli and golgi apparatus. -
Gene Info — PARP10
Entrez GeneID
84875Protein Accession#
Q53GL7Gene Name
PARP10
Gene Alias
FLJ14464
Gene Description
poly (ADP-ribose) polymerase family, member 10
Omim ID
609564Gene Ontology
HyperlinkGene Summary
Poly(ADP-ribose) polymerases (PARPs), such as PARP10, regulate gene transcription by altering chromatin organization by adding ADP-ribose to histones. PARPs can also function as transcriptional cofactors (Yu et al., 2005 [PubMed 15674325]).[supplied by OMIM
Other Designations
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