HINT3 polyclonal antibody
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More Files
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Specifications
Product Description
Rabbit polyclonal antibody raised against recombinant HINT3.
Immunogen
Recombinant protein corresponding to amino acids of human HINT3.
Sequence
MAEEQVNRSAGLAPDCEASATAETTVSSVGTCEAAAKSPEPKDYDSTCVFCRIAGRQDPGTELLHCENEDLICFKDIKPAATHHYLVVP
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with HINT3 polyclonal antibody (Cat # PAB22086) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human colon with HINT3 polyclonal antibody (Cat # PAB22086) shows moderate cytoplasmic positivity in glandular cells at 1:50-1:200 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with HINT3 polyclonal antibody (Cat # PAB22086) at 1-4 ug/mL dilution shows positivity in cytoplasm and mitochondria. -
Gene Info — HINT3
Entrez GeneID
135114Protein Accession#
Q9NQE9Gene Name
HINT3
Gene Alias
FLJ33126, FLJ99898, HINT4, MGC22976
Gene Description
histidine triad nucleotide binding protein 3
Omim ID
609998Gene Ontology
HyperlinkGene Summary
Histidine triad proteins, such as HINT3, are nucleotide hydrolases and transferases that act on the alpha-phosphate of ribonucleotides (Brenner, 2002 [PubMed 12119013]).[supplied by OMIM
Other Designations
HIT-like protein|OTTHUMP00000017150
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