NUP153 polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against recombinant NUP153.
Immunogen
Recombinant protein corresponding to amino acids of human NUP153.
Sequence
GQNREQRESGFSYPNFSLPAANGLSSGVGGGGGKMRRERTRFVASKPLEEEEMEVPVLPKISLPITSSSLPTFNFSSPEITTSSPSPINSSQALTNKVQMTSP
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:200-1:500)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with NUP153 polyclonal antibody (Cat # PAB22081) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cerebellum with NUP153 polyclonal antibody (Cat # PAB22081) shows strong nuclear and nuclear membranous positivity at 1:200-1:500 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with NUP153 polyclonal antibody (Cat # PAB22081) at 1-4 ug/mL dilution shows positivity in nuclear membrane. -
Gene Info — NUP153
Entrez GeneID
9972Protein Accession#
P49790Gene Name
NUP153
Gene Alias
HNUP153, N153
Gene Description
nucleoporin 153kDa
Omim ID
603948Gene Ontology
HyperlinkGene Summary
Nuclear pore complexes are extremely elaborate structures that mediate the regulated movement of macromolecules between the nucleus and cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are pore complex-specific glycoproteins characterized by cytoplasmically oriented O-linked N-acetylglucosamine residues and numerous repeats of the pentapeptide sequence XFXFG. The protein encoded by this gene has three distinct domains: a N-terminal region within which a pore targeting domain has been identified, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats. [provided by RefSeq
Other Designations
OTTHUMP00000039309|nuclear pore complex protein hnup153
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