MINA polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MINA.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human MINA.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of MINA polyclonal antibody (Cat # PAB2203) pre-incubated with and without blocking peptide (BP) in Jurkat cell line lysate. MINA (C-term) (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with MINA polyclonal antibody (Cat # PAB2203) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. -
Gene Info — MINA
Entrez GeneID
84864Protein Accession#
NP_116167;Q8IUF8Gene Name
MINA
Gene Alias
DKFZp762O1912, FLJ14393, MDIG, MINA53, NO52
Gene Description
MYC induced nuclear antigen
Gene Ontology
HyperlinkGene Summary
MINA is a c-Myc (MYC; MIM 190080) target gene that may play a role in cell proliferation or regulation of cell growth. (Tsuneoka et al., 2002 [PubMed 12091391]; Zhang et al., 2005 [PubMed 15897898]).[supplied by OMIM
Other Designations
mineral dust induced gene protein|myc-induced nuclear antigen, 53 kDa
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Interactome
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Publication Reference
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NO66, a highly conserved dual location protein in the nucleolus and in a special type of synchronously replicating chromatin.
Eilbracht J, Reichenzeller M, Hergt M, Schnolzer M, Heid H, Stohr M, Franke WW, Schmidt-Zachmann MS.
Molecular Biology of the Cell 2004 Apr; 15(4):1816.
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Increased expression of a Myc target gene Mina53 in human colon cancer.
Teye K, Tsuneoka M, Arima N, Koda Y, Nakamura Y, Ueta Y, Shirouzu K, Kimura H.
The American Journal of Pathology 2004 Jan; 164(1):205.
Application:IF, WB, Human, HeLa, Human colon cancer, SW-620 cells.
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A novel myc target gene, mina53, that is involved in cell proliferation.
Tsuneoka M, Koda Y, Soejima M, Teye K, Kimura H.
The Journal of Biological Chemistry 2002 Jun; 277(38):35450.
Application:IF, Human, Rat, 3Y1, HeLa cells.
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NO66, a highly conserved dual location protein in the nucleolus and in a special type of synchronously replicating chromatin.
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