APIP polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant APIP.
Immunogen
Recombinant protein corresponding to amino acids of human APIP.
Sequence
NTPEEKDLKDRMAHAMNEYPDSCAVLVRRHGVYVWGETWEKAKTMCECYDYLFDIAVSMKKVGLDPSQLPVGENGIV
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:250-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with APIP polyclonal antibody (Cat # PAB21948).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human kidney with APIP polyclonal antibody (Cat # PAB21948) shows cytoplasmic and nuclear positivity in tubular cells. -
Gene Info — APIP
Entrez GeneID
51074Protein Accession#
Q96GX9Gene Name
APIP
Gene Alias
APIP2, CGI-29, CGI29, MMRP19, dJ179L10.2
Gene Description
APAF1 interacting protein
Gene Ontology
HyperlinkGene Summary
APIP is an APAF1 (MIM 602233)-interacting protein that acts as a negative regulator of ischemic/hypoxic injury (Cho et al., 2004 [PubMed 15262985]).[supplied by OMIM
Other Designations
APAF1-interacting protein
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Interactome
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Pathway
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