C12orf53 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant C12orf53.
Immunogen
Recombinant protein corresponding to amino acids of human C12orf53.
Sequence
DRSQKRRRPSGQQGALRQEESQQPLTDLSPAGVTVLGAFGDSPTPTPDHEEPRGGPRPGMPHPKGAPAFQLNR
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate).
Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with C12orf53 polyclonal antibody (Cat # PAB21925).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cerebral cortex with C12orf53 polyclonal antibody (Cat # PAB21925) shows distinct cytoplasmic and nuclear positivity in neurons. -
Gene Info — C12orf53
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Interactome
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Publication Reference
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Complex formation of APP with GABAB receptors links axonal trafficking to amyloidogenic processing.
Dinamarca MC, Raveh A, Schneider A, Fritzius T, Früh S, Rem PD, Stawarski M, Lalanne T, Turecek R, Choo M, Besseyrias V, Bildl W, Bentrop D, Staufenbiel M, Gassmann M, Fakler B, Schwenk J, Bettler B.
Nature Communications 2019 Mar; 10(1):1331.
Application:IF, IP, WB-Ti, WB-Tr, Human, Mouse, HEK 293 cells, Mouse brains.
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Complex formation of APP with GABAB receptors links axonal trafficking to amyloidogenic processing.
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