TRDMT1 polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of TRDMT1 polyclonal antibody (Cat # PAB2186) in Jurkat cell lysate (Lane A) and mouse liver tissue lysate (Lane B) . Dnmt2 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of TRDMT1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 353-383 at C-terminus of human TRDMT1.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of TRDMT1 polyclonal antibody (Cat # PAB2186) in Jurkat cell lysate (Lane A) and mouse liver tissue lysate (Lane B) . Dnmt2 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — TRDMT1
Entrez GeneID
1787Protein Accession#
O14717Gene Name
TRDMT1
Gene Alias
DNMT2, M.HsaIIP, PuMet, RNMT1
Gene Description
tRNA aspartic acid methyltransferase 1
Omim ID
602478Gene Ontology
HyperlinkGene Summary
This gene encodes a protein responsible for the methylation of aspartic acid transfer RNA. While similar in sequence and structure to DNA cytosine methyltransferases, this gene is distinct and highly conserved in its function among taxa. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
DNA (cytosine-5-)-methyltransferase 2|DNA MTase homolog HsaIIP|DNA methyltransferase homolog HsaIIP|DNA methyltransferase-2|OTTHUMP00000019228|OTTHUMP00000045198|tRNA aspartic acid methyltransferase 1 variant 1|tRNA aspartic acid methyltransferase 1 varia
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Interactome
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Pathway
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Disease
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Publication Reference
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The human Dnmt2 has residual DNA-(cytosine-C5) methyltransferase activity.
Hermann A, Schmitt S, Jeltsch A.
The Journal of Biological Chemistry 2003 Aug; 278(34):31717.
Application：Incubated, Recombinant protein.
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Five novel alternatively spliced transcripts of DNA (cytosine-5) methyltransferase 2 in human peripheral blood leukocytes.
Franchina M, Hooper J, Kay PH.
The International Journal of Biochemistry & Cell Biology 2001 Nov; 33(11):1104.
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Structure of human DNMT2, an enigmatic DNA methyltransferase homolog that displays denaturant-resistant binding to DNA.
Dong A, Yoder JA, Zhang X, Zhou L, Bestor TH, Cheng X.
Nucleic Acids Research 2001 Jan; 29(2):439.
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The human Dnmt2 has residual DNA-(cytosine-C5) methyltransferase activity.
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