RAB14 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant RAB14.
Immunogen
Recombinant protein corresponding to amino acids of human RAB14.
Sequence
GQERFRAVTRSYYRGAAGALMVYDITRRSTYNHLSSWLTDARNLTNPNTVIILIGNKADLEAQRDVTYEEAKQFAEENGLLFLEASAKTGENVEDAFLEAAKKIYQNIQDGSLDLNAAESGVQHKPSAPQGGRLTSEPQPQ
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:10-1:20)
Western Blot (1:250-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with RAB14 polyclonal antibody (Cat # PAB21798) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human heart muscle with RAB14 polyclonal antibody (Cat # PAB21798) shows distinct positivity in intercalated discs at 1:10-1:20 dilution. -
Gene Info — RAB14
Entrez GeneID
51552Protein Accession#
P61106Gene Name
RAB14
Gene Alias
FBP, RAB-14
Gene Description
RAB14, member RAS oncogene family
Gene Ontology
HyperlinkGene Summary
RAB14 belongs to the large RAB family of low molecular mass GTPases that are involved in intracellular membrane trafficking. These proteins act as molecular switches that flip between an inactive GDP-bound state and an active GTP-bound state in which they recruit downstream effector proteins onto membranes (Junutula et al., 2004 [PubMed 15004230]).[supplied by OMIM
Other Designations
F protein-binding protein 1|GTPase Rab14|OTTHUMP00000022008|bA165P4.3 (member RAS oncogene family)|small GTP binding protein RAB14
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