PRMT7 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of PRMT7 (arrow) using PRMT7 polyclonal antibody (Cat # PAB2178).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRMT7 gene (Lane 2).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PRMT7.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PRMT7.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of PRMT7 (arrow) using PRMT7 polyclonal antibody (Cat # PAB2178).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRMT7 gene (Lane 2).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — PRMT7
Entrez GeneID
54496Protein Accession#
NP_061896Gene Name
PRMT7
Gene Alias
FLJ10640, KIAA1933
Gene Description
protein arginine methyltransferase 7
Omim ID
610087Gene Ontology
HyperlinkGene Summary
Arginine methylation is an apparently irreversible protein modification catalyzed by arginine methyltransferases, such as PMT7, using S-adenosylmethionine (AdoMet) as the methyl donor. Arginine methylation is implicated in signal transduction, RNA transport, and RNA splicing (Miranda et al., 2004 [PubMed 15044439]).[supplied by OMIM
Other Designations
protein arginine N-methyltransferase 7
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Interactome
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Publication Reference
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PRMT7, a new protein arginine methyltransferase that synthesizes symmetric dimethylarginine.
Lee JH, Cook JR, Yang ZH, Mirochnitchenko O, Gunderson SI, Felix AM, Herth N, Hoffmann R, Pestka S.
The Journal of Biological Chemistry 2005 Feb; 280(5):3656.
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PRMT7 is a member of the protein arginine methyltransferase family with a distinct substrate specificity.
Miranda TB, Miranda M, Frankel A, Clarke S.
The Journal of Biological Chemistry 2004 May; 279(22):22902.
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The novel human protein arginine N-methyltransferase PRMT6 is a nuclear enzyme displaying unique substrate specificity.
Frankel A, Yadav N, Lee J, Branscombe TL, Clarke S, Bedford MT.
The Journal of Biological Chemistry 2001 Nov; 277(5):3537.
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PRMT7, a new protein arginine methyltransferase that synthesizes symmetric dimethylarginine.
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