DNAJC24 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant DNAJC24.
Immunogen
Recombinant protein corresponding to amino acids of human DNAJC24.
Sequence
LQRCEDDLRNVGPVDAQVYLEEMSWNEGDHSFYLSCRCGGKYSVSKDEAEEVSLISCDTCSLIIELLHY
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:250-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with DNAJC24 polyclonal antibody (Cat # PAB21766).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human placenta with DNAJC24 polyclonal antibody (Cat # PAB21766) shows strong membranous positivity in trophoblastic cells. -
Gene Info — DNAJC24
Entrez GeneID
120526Protein Accession#
Q6P3W2Gene Name
DNAJC24
Gene Alias
DPH4, JJJ3, ZCSL3
Gene Description
DnaJ (Hsp40) homolog, subfamily C, member 24
Omim ID
611072Gene Ontology
HyperlinkGene Summary
Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH4 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).[supplied by OMIM
Other Designations
1700030A21Rik|DPH4 homolog (JJJ3, S. cerevisiae)|DPH4, JJJ3 homolog|zinc finger, CSL domain containing 3|zinc finger, CSL-type containing 3
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