PRMT1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PRMT1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human PRMT1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of PRMT1 (arrow) using rabbit PRMT1 polyclonal antibody (Cat # PAB2168). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRMT1 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — PRMT1
Entrez GeneID
3276Protein Accession#
Q99873Gene Name
PRMT1
Gene Alias
ANM1, HCP1, HRMT1L2, IR1B4
Gene Description
protein arginine methyltransferase 1
Omim ID
602950Gene Ontology
HyperlinkGene Summary
The PRMT1 gene encodes a protein arginine methyltransferase that functions as a histone methyltransferase specific for H4 (see MIM 602822).[supplied by OMIM
Other Designations
HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 2|HMT1 hnRNP methyltransferase-like 2|interferon receptor 1-bound protein 4|protein arginine N-methyltransferase 1
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Interactome
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Disease
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Publication Reference
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Genomic organization, physical mapping, and expression analysis of the human protein arginine methyltransferase 1 gene.
Scorilas A, Black MH, Talieri M, Diamandis EP.
Biochemical and Biophysical Research Communications 2000 Nov; 278(2):349.
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Crystal structure of the conserved core of protein arginine methyltransferase PRMT3.
Zhang X, Zhou L, Cheng X.
The EMBO Journal 2000 Jul; 19(14):3509.
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Identification and characterization of two putative human arginine methyltransferases (HRMT1L1 and HRMT1L2).
Scott HS, Antonarakis SE, Lalioti MD, Rossier C, Silver PA, Henry MF.
Genomics 1998 Mar; 48(3):330.
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Genomic organization, physical mapping, and expression analysis of the human protein arginine methyltransferase 1 gene.
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