RP5-1000E10.4 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant RP5-1000E10.4.
Immunogen
Recombinant protein corresponding to amino acids of human RP5-1000E10.4.
Sequence
ESLVDQSAALHRRVAAMREAGTALPDQYQEDASDMKDMSKYKPHILLSQENTQIRDLQQENRELWISLEEHQDALEL
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:200-1:500)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with RP5-1000E10.4 polyclonal antibody (Cat # PAB21658) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human small intestine with RP5-1000E10.4 polyclonal antibody (Cat # PAB21658) strong cytoplasmic positivity in glandular cells at 1:200-1:500 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with RP5-1000E10.4 polyclonal antibody (Cat # PAB21658) at 1-4 ug/mL dilution shows positivity in focal adhesions. -
Gene Info — RP5-1000E10.4
Entrez GeneID
80143Protein Accession#
Q9BRV8Gene Name
RP5-1000E10.4
Gene Alias
DKFZp686A0768, FLJ21168, SIKE
Gene Description
suppressor of IKK epsilon
Omim ID
611656Gene Ontology
HyperlinkGene Summary
SIKE interacts with IKK-epsilon (IKBKE; MIM 605048) and TBK1 (MIM 604834) and acts as a suppressor of TLR3 (MIM 603029) and virus-triggered interferon activation pathways (Huang et al., 2005 [PubMed 16281057]).[supplied by OMIM
Other Designations
OTTHUMP00000013884|OTTHUMP00000013885
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